Reconstruction Of Hippocampus Ca1 Stratum Pyramidale, Pv+ Basket Cell

title : Reconstruction of hippocampus CA1 cell morphologies specimen : Rattus norvegicus sex : male weight : 100-200 g This neuron was recorded and filled with biocytin in a 450-500 µm thick coronal slice of adult rat hippocampus, using sharp electrodes (for methods see PMID: 11807843). The fixed slice was resectioned at 50-60 µm and the cell reconstructed with Neurolucida using a 100x oil immersion objective.标题：海马体CA1神经元形态重构 实验标本：褐家鼠（Rattus norvegicus） 性别：雄性 体重：100~200克 本神经元于成年大鼠海马体450~500 μm厚的冠状脑片中，采用尖锐电极完成胞内记录并填充生物胞素（biocytin），具体实验方法详见PMID: 11807843。固定后的脑片被切片至50~60 μm厚度，随后借助100×油浸物镜，通过Neurolucida软件完成该细胞的形态重构。