Deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in MEFs

The goal of this study is to assess the effect of the deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in mouse embryonic fibroblasts. Overall design: We used RTEL1F/F mouse embryonic fibroblasts that upon infection with Cre adenoparticles delete Rtel1 and upon addition of doxycycline overexpress WT RNaseH1GFP (Rtel1F/F;WT RNH1-GFP MEFs). Rtel1F/F;WT RNH1-GFP MEFs were infected with GFP or Cre-GFP adenovirus, after 48 h doxycycline (2 µg/ml) was added and cells were collected after 48 h. RNA:DNA hybrids were pulled down using the DRIP protocol and DNA was isolated and send for sequencing.