Marking Fish with Fluorochrome Dyes

Marking fish with fluorochrome dyes has the potential to produce detectable marks in otoliths, scales, fin rays, fin spines, and other bony structures based on differential staining of chemical dyes, and it is a widely used technique for mass-marking fish that included both teleosts and elasmobranchs. This technique, which has relatively economic advantages and simple marking process, can be applied to fish of all ages, and these fluorescent marks can be retained for several months and even years. Up to now, >200 fish species have been successfully marked by this marking technique. Fluorescent labeling is a practical and available marking technique for large-scale marking hatchery-reared fish. The methodology of fluorescent labeling is reviewed, including fluorochrome labeling dyes, application method, detection, and evaluation strategies. Aspects of practical applications, limitations, and supervision of fluorescent labeling are also discussed.

利用荧光染料(fluorochrome dyes)标记鱼类，可通过化学染料的差异染色在耳石(otoliths)、鳞片、鳍条(fin rays)、鳍棘(fin spines)及其他骨组织中形成可检测的标记；该技术是当前广泛应用于真骨鱼类(teleosts)与软骨鱼类(elasmobranchs)的大规模鱼类标记技术。该技术兼具成本优势与操作简便的特点，可适用于各年龄段的鱼类，且此类荧光标记可留存数月乃至数年之久。迄今已有超过200种鱼类通过该标记技术成功完成标记。荧光标记技术是一种适用于规模化标记孵化繁育鱼类(hatchery-reared fish)的实用且可行的标记手段。本文综述了荧光标记技术的方法学，涵盖荧光标记染料、应用方法、检测手段及评估策略等内容。此外还探讨了该技术的实际应用、局限性及应用监管等相关方面。