The α/β hydrolase DWARF14-Like 2 regulates growth and development in Arabidopsis thaliana

To understand the role of DLK2 in regulating plant growth and development, we performed the comparative transcriptome profiling WT and DLK2-OE1 seedlings. 11-day-old WT and DLK2-OE1 seedlings were transferred from 1/2 Murashige and Skoog (MS) plates to soil and grown for 13 additional day. The rosette leaves of this 24-day-old seedlings were frozen in liquid nitrogen immediately. Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer’s protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo (dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB#7530,New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end repaired. A base added, and ligated to Illumina sequencing adapters. The ligation reaction was purified with the AMPure XP Beads (1.0X).And polymerase chain reaction (PCR) amplified. The resulting cDNA library was sequenced using Illumina Novaseq6000.