Running times.

Times, in hours, minutes and seconds, taken by CStone to assemble datasets used in this study on Windows 10 running on 32 cores (AMD Ryzen Threadripper 2990WX @ 3.00GHz) and 128GB of ram, as well as on Ubuntu 20.04 running on 24 cores (Intel Xeon(R) CPU E5-2697 v2 @ 2.70GHz) and 64GB of ram. For simulated datasets “Effective transcriptome size” refers to the cDNA reference transcripts from which the reads were simulated, whilst for real data it is the (unknown) number of expressed genes within the adults that were sequenced.