Table 1. Electrophoretic conditions for 10 enzymatic systems assayed for Gymnotus inaequilabiatus, G. sylvius and G in Genetic variability in three species of Gymnotus Linnaeus, 1758 (Gymnotiformes: Gymnotidae) from Caracu stream of the upper Paraná River basin, Brazil

Table 1. Electrophoretic conditions for 10 enzymatic systems assayed for Gymnotus inaequilabiatus, G. sylvius and G. pantanal from Caracu stream, Paraná State, Brazil. EnzymeTissueBufferTimeReferencesAAT, EST, ADH, GDH, SODLiver, KidneyTris/borate/EDTA 0.045/0.025/0.001M, pH 8.67 hoursBoyer et al. (1963)G3PDH, LDH, SORBLiver, KidneyTris/citrate 0.009/0.003M, pH 7.014 hoursShaw & Prasad (1970)IDH, MDHLiver, Muscle, GillTris/citrate 0.009/0.003M, pH 7.014 hoursShaw & Prasad (1970)表1 巴西巴拉那州卡拉库溪的不均衡裸背电鳗（Gymnotus inaequilabiatus）、G. sylvius 及G. pantanal 三种鱼类所检测的10种酶系统电泳条件 酶类 组织 缓冲液 电泳时长 参考文献 AAT、EST、ADH、GDH、SOD 肝脏、肾脏 Tris/硼酸盐/EDTA 0.045/0.025/0.001M，pH 8.6 7小时 Boyer等（1963） G3PDH、LDH、SORB 肝脏、肾脏 Tris/柠檬酸盐 0.009/0.003M，pH 7.0 14小时 Shaw & Prasad（1970） IDH、MDH 肝脏、肌肉、鳃 Tris/柠檬酸盐 0.009/0.003M，pH 7.0 14小时 Shaw & Prasad（1970）