Cytotoxic activity of betulinic acid and ursolic acid againts T47D breast cancer cells

Uncaria nervosa Elmer is an Indonesian herbal plant that is traditionally used for breast cancer. The results of phytochemical screening contained alkaloids, flavonoids, and terpenoids in the ethanol extract of this plant. Based on literature searches, reports regarding the bioactive compounds responsible for breast cancer have not been found. This study aims to determine the metabolite profiling of ethanol extract, the isolation, characterization of bioactive compounds, and their bioactivity in T47D breast cancer cells. The ethanol extract of Uncaria nervosa Elmer leaves contains nine compounds consisting of alkaloids, terpenoids, and fatty acid. The bioactive compounds that were successfully isolated were betulinic acid, and ursolic acid, with IC50 values of ˃100 and 14,70±4,50 µg/ml, respectively.显脉钩藤（Uncaria nervosa Elmer）是一种印度尼西亚药用植物，传统上用于乳腺癌的治疗。该植物乙醇提取物的植物化学筛选结果显示，其含有生物碱、黄酮类及萜类成分。经文献调研发现，目前尚未见关于其抗乳腺癌活性成分的相关报道。本研究旨在明确该植物乙醇提取物的代谢物谱，完成活性成分的分离与表征，并评估其在T47D乳腺癌细胞中的生物活性。显脉钩藤叶片的乙醇提取物共含有9种成分，涵盖生物碱、萜类及脂肪酸类。本研究成功分离得到的活性成分为白桦脂酸（betulinic acid）与熊果酸（ursolic acid），二者的半数抑制浓度（IC50）分别为＞100及14.70±4.50 µg/ml。