Transfection values (RLU/mg of protein) of RTNs nebulised through the NGI with either Aeroneb® Pro, PARI-LC Plus or AeroEclipse II BAN.

Thr = throat; S1–S8 = stages 1–8 of the NGI, S8 = micro-orifice collector. All three nebulisers connected to the NGI were operated for 10 min at an air flow rate of 15 L/min as described in methods. The transfection results are average values of three independent experiments, each one was carried out in quadruplicate, and the error bars represent the standard error of the mean.