Spectral decomposition coefficients for spatiotemporal gene expression pattern identification in Bacillus subtilis swarm development

Spectral decomposition coefficients, spatiotemporal gene expression pattern identification and multidimensional scaling coordinates of spatiotemporal transcriptomics data and image analysis results used to create Figure 2 in the scientific article "Simultaneous spatiotemporal transcriptomics and microscopy of <em>Bacillus subtilis</em> swarm development reveal cooperation across generations" by the following authors: Hannah Jeckel*, Kazuki Nosho*, Konstantin Neuhaus, Alasdair D. Hastewell, Dominic J. Skinner, Dibya Saha, Niklas Netter, Nicole Paczia, Jörn Dunkel, Knut Drescher. The symbol "*" indicates an equal contribution. This data consists of two excel sheets, one for the transcriptomics data and one for image analysis results (physical properties). Genes were ranked according to a spatiotemporal information score defined in the publication described above. For each gene, its name and ID (derived from locus tag) are given as identifiers. For information of reference genome used for mapping and convention on how gene names are chosen, see https://drescherlab.org/data/swarm-transcriptome/. Other columns in the sheet represent the spatiotemporal information score, assigned spatiotemporal pattern number, decomposition coefficient, multidimensional scaling coordinates and gene function. Physical properties are measured from short microscopy videos and defined in the publication mentioned above. This excel sheet contains property name, spatiotemporal information score, spectral decomposition coefficients and multidimensional scaling coordinates.本数据集包含光谱分解系数、时空基因表达模式识别结果以及多维缩放坐标（multidimensional scaling coordinates），为科学论文《枯草芽孢杆菌（*Bacillus subtilis*）群体运动发育的同步时空转录组学与显微成像研究揭示跨代协同作用》（作者：Hannah Jeckel*、Kazuki Nosho*、Konstantin Neuhaus、Alasdair D. Hastewell、Dominic J. Skinner、Dibya Saha、Niklas Netter、Nicole Paczia、Jörn Dunkel、Knut Drescher，其中*标识同等贡献作者）中用于制作图2的时空转录组学数据与图像分析结果。本数据集包含两个Excel工作表，其一对应转录组学数据，其二对应图像分析结果（物理属性）。研究人员依据上述论文中定义的时空信息评分对基因进行排序，每个基因均以基因名称与（源自基因座位标签（locus tag）的）基因ID作为标识符。关于用于序列比对的参考基因组信息以及基因命名规范，请参阅https://drescherlab.org/data/swarm-transcriptome/。该转录组学数据工作表的其余列分别包含时空信息评分、分配的时空模式编号、分解系数、多维缩放坐标以及基因功能信息。物理属性参数通过短时长显微视频测量得到，其定义已在上述提及的论文中阐明。该图像分析结果Excel工作表包含属性名称、时空信息评分、光谱分解系数以及多维缩放坐标。