T-cell receptors targeting distinct FLT3-D835Y neoantigens display differential reactivity toward acute myeloid leukemia_flow_cytometry_data_4

Figure 4C / Figure S8C: FACS staining of FLT3-B8 TCR-T cells activation assay with HLA-B*08:01-transduced K562 cells loaded with titrated concentrations of FLT3-D835Y neopeptide FGLARYIM or wildtype peptide FGLARDIM. Fluorochromes: Zombie Red = viability dye, Pacific Blue = CD8, BV711 = mTCRcbeta, APC = CD137. Figure 5H / Figure S11B: FACS staining of lysis assay of MOLM13-RES-AC cells (pre-treated with Crenolanib) upon coincubation with FLT3-A2 TCR-T cells. Fluorochromes: Zombie Red = viability dye, Pacific Blue = CD3 / CD8, FITC = HLA-A*02, PE = CD33. Figure 5I: FLT3 staining of MOLM13-RES-AC cells pre-treated with Crenolanib. Fluorochromes: Zombie Red = viability dye, BV650 = FLT3, FITC = HLA-A*02.