Isoreticular, interpenetrating co-crystal of protein variant Replication Initiator Protein REPE54 (L53G,Q54G,E55G) and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence.

Isoreticular, interpenetrating co-crystal of protein variant Replication Initiator Protein REPE54 (L53G,Q54G,E55G) and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence. Descriptor: DNA (5'-D(A*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*P*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*G)-3'), DNA (5'-D(A*GP*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*P*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*G)-3'), MAGNESIUM ION, ... Authors: Orun, A.R, Snow, C.D. Deposit date: 2022-05-06 Release date: 2023-05-17 Last modified: 2023-11-01 Method: X-RAY DIFFRACTION (3.15 Å) Cite: Modular Protein-DNA Cocrystals as Precise, Programmable Assembly Scaffolds. Acs Nano, 17, 2023

本数据集为同网状互穿共晶体，由蛋白质变体复制起始蛋白（Replication Initiator Protein）REPE54（L53G、Q54G、E55G位点突变体）与包含同源REPE54序列及额外富G-C序列的对称扩展双链体（31聚体）共同构成。序列描述：DNA（5'-D(A*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*P*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*G)-3'）、DNA（5'-D(A*GP*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*P*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*G)-3'）、镁离子（Magnesium Ion）等。作者：Orun, A.R.、Snow, C.D. 入库日期：2022-05-06 发布日期：2023-05-17 最后修改日期：2023-11-01 实验方法：X射线衍射（分辨率3.15 Å） 引用文献：《模块化蛋白-DNA共晶体作为精准可编程组装支架》，ACS Nano，17卷，2023年