Replication Data "The Ameliorative Effect of Atorvastatin on Fertility and Testicular Oxidant/Antioxidant System of HFD-Fed Male Albino Rats"

Description of data This data investigates the effect of 4 weeks high fat diet (HFD) administration to male albino rats and putative alleviation potentials of atorvastatin (AS) administered in the last 2 weeks of the experiment in dose 5 mg/kg/day. Serum atherogenic index (AX), total testosterone, and TSH levels were estimated as well as the testicular levels and activities of MDA, GSH and GR, SOD, and GST respectively. Supplementing high fat diet to rats (HFD) exerted a significant increase in serum atherogenic index (AX) by 31% relative to normal control (NC) one. However, administering atorvastatin (AS) ameliorated this effect by decreasing serum AX by 18 % relative to HFD-fed group’s one (Table 3). Regarding to serum total testosterone, HFD caused a profound decrease of this level by 62 % in comparison to CN, while AS alleviate this bad effect by increasing the level by 92 %. However, neither HFD nor AS showed significant changes to serum TSH (table 3). Estimating the testicular level of malondialdehyde (MDA) as a marker of lipid peroxidation and oxidative stress, HFD exerted a tremendous increase to 113% of this level. Fortunately, AS exhibited a profound amelioration by causing a 54 % decrease relative to HFD group (Table 4). The reduced form of glutathione (GSH) content in rat testes was profoundly changed by HFD supplementation reaching to 87 % increase, while AS supplementation caused 30 % decrease (Table 4). The testicular activity of glutathione reductase (GR) was decreased by 42 % relative to CN but AS increased this activity by 112 % comparable to HFD group (Table 5). By determining the testicular activity of superoxide dismutase (SOD), there were moderate increase and decrease by 14 % and 4 % as a result of administering HFD and AS respectively (Table 5). On the other side, glutathione-S transferase activity in testicular tissue showed modest increase by 11 % as a result of HFD supplementation. Surprisingly, AS exerted an insignificant change in comparison with HFD group (Table 5).