Figure 7 - A549 (53BP1+PCNA) - Control Cells - Raw LIF

A549 Lung Adenocarcinoma cells were engineered to co-express a PCNA chromobody tagged with mNeonGreen (green) and a truncated version of 53BP1 tagged with mApple (red). Cells were synchronised with 1mM thymidine and pulsed with 5µg/ml cisplatin for 2 hr prior to release to enrich cells in early S phase. Cells were then imaged using a Leica SP8 confocal microscopy, using HyD detectors a WLL and 63X 1.4 NA objective. A 10-µm-thick z-stack (1 µm slice, 1AU) was taken every 30 min for 72 hr, with the maximum projection raw LIF and compressed MP4 files included here.<br>This data relates to Figure 7 of the published article:Rajal AG, Marzec KA, McCloy RA, Nobis M, Chin V, Hastings JF, Lai K, Kennerson M, Hughes WE, Vaghjiani V, Timpson P, Cain JE, Watkins DN, Croucher DR, Burgess A (2021) A non-genetic, cell cycle dependent mechanism of platinum resistance in lung adenocarcinoma. Elife 10:e65234. https://doi.org/10.7554/elife.65234