Crosstalk assessment for multi-colour immunofluorescence of Histone 3 and Polymerase II post-translational modifications in pluripotent zebrafish embryos

Microscopy images recorded to assess the extent of crosstalk from the detection channels of H3K27ac and recruited RNA polymerase II (Serine 5 phosphorylation of the C-terminal domain heptad repeat of subunit 1) to the detection channel of elongating RNA polymerase II (Serine 5 phosphorylation of the C-terminal domain heptad repeat of subunit 1). Sample preparation and image recording was carried out jointly by Süheyla Eroğlu-Kayikci, Elisa Kämmer, and Lennart Hilbert.本数据集收录了用于评估H3K27ac与募集的RNA聚合酶II（亚基1 C端结构域七肽重复序列的丝氨酸5磷酸化）的检测通道对延伸型RNA聚合酶II（亚基1 C端结构域七肽重复序列的丝氨酸5磷酸化）检测通道的串扰程度的显微图像。样本制备与图像采集工作由Süheyla Eroğlu-Kayikci、Elisa Kämmer以及Lennart Hilbert共同完成。