INS-1E PBC
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Tilt Series Date: 2018-07-15 Data Taken By: Stephen Carter Species / Specimen: Rat INS-1E pancreatic beta cells Strain: Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x. Microscope: Caltech Titan Krios Acquisition Software: SerialEM Upload Method: reupload_downloaded Processing Software Used: IMOD Collaborators and Roles: Ray Stevens Purification / Growth Conditions / Treatment: INS-1E cells grown on gold finder grids, stimulated with glucose. Sample Preparation: Manual Blotting
倾斜序列采集日期:2018-07-15 数据采集者:Stephen Carter 物种/标本:大鼠INS-1E胰腺β细胞(pancreatic beta cells) 细胞株:INS-1E 倾斜序列设置:单轴倾斜模式,倾斜范围为(-60.0°, 60.0°),步长为1°,采用恒定角度增量,电子剂量为120.0 eV/Ų,欠焦量为-10.0 μm,放大倍数为34000倍 显微镜:加州理工学院(Caltech)Titan Krios(Titan Krios) 采集软件:SerialEM(SerialEM) 上传方式:重新上传下载文件(reupload_downloaded) 使用的图像处理软件:IMOD(IMOD) 合作者及职责:Ray Stevens 纯化/培养条件/处理方式:将INS-1E细胞接种于金载网(gold finder grids)上,并用葡萄糖进行刺激处理 样品制备:手动滤纸吸干法(Manual Blotting)



