Chromatin conformation identification by Cut-C

Understanding of the 3D structure of the genome is essential to decipher the detailed regulatory mechanisms of gene expression. Here we present CUT-C, a method that combines the antibody mediated cleavage by tethered nuclease with the chromosome conformation capture technique to identify chromatin interactions mediated by a protein of interest. CUT-C identifies protein-centric chromatin conformation along with the genome wide distribution of target proteins. Overall design: We developed a method to identify protein centric chromatin conformation based on micro-c. Unlike micro-c, DNA is cleaved by Mnase thethered by target proteins. Cleaved regions are identified by next generation sequencing.