Urinary single cell profiling captures cellular diversity of the kidney

We performed single cell transcriptomic analysis on 17 urine samples obtained from five subjects at two different occasions using both spot and 24-hour urine collection. In addition, we used a combined spot urine samples of five healthy individuals as a control sample. We sequenced a total of 71,667 cells. After quality control and downstream analysis, we found that epithelial cells were the most common cell types in the urine. We were also able to identify most kidney cell types in the urine, such as podocyte, proximal, and collecting duct (CD), in addition to macrophages, monocytes and lymphocytes. Single cell RNA sequencing for 17 urine samples collected from 5 DKD patients of TRIDENT cohort and a pooled urine sample of five healthy individuals