Multilocus Rickettsia typing and barcoding of BOLD Rickettsia contaminants

COI sequences alone provides an impression of the frequency with which Rickettsia associates are found in barcoding studies. However, they have limited value in describing the diversity of the Rickettsia found. To provide further insight into the diversity of Rickettsia using a multilocus approach, we obtained 186 DNA extracts from the archive at the Centre for Biodiversity Genomics (University of Guelph, Canada). We then screened multiple Rickettsia genes via PCR (gltA, 16S, 17KDa) to create strain types. Accession numbers for both Rickettsia and host sequences are provided in this dataset.

仅通过细胞色素c氧化酶亚基I（COI）序列，便可获知立克次体（Rickettsia）关联类群在DNA条形码研究中的检出频率。但这类序列在刻画所检出立克次体的多样性时价值有限。为借助多基因座分析策略进一步解析立克次体的多样性，我们从加拿大圭尔夫大学生物多样性基因组学中心（Centre for Biodiversity Genomics, University of Guelph）的存档样本中获取了186份DNA提取物。随后通过聚合酶链式反应（PCR）对多个立克次体基因（gltA、16S、17KDa）进行扩增筛选，以构建菌株分型。本数据集提供了立克次体及宿主序列的登录号（Accession Numbers）。