Unmasking of Protein Phosphatase 2 Regulatory Subunit B as a crucial factor in the development and progression of dilated cardiomyopathy. Unmasking of Protein Phosphatase 2 Regulatory Subunit B as a crucial factor in the development and progression of dilated cardiomyopathy

The PPP2R5D encodes for a Protein phosphatase 2A (PP2A), one of the four major Ser/Thr phosphatases negatively controlling cell growth and division. PP2A is consisting of a common heteromeric core enzyme with a catalytic subunit and a B regulatory subunit that might control the substrate selectivity and catalytic activity. We investigated the potential role of Ppp2r5d for the cardiomyocyte (cultured mouse cardiomyocytes). Overall design: The interfering RNA against Ppp2r5d (Case) or the negative control siRNA (Control) were transfected into MCMs（mouse cardiomyocytes） with Lipofectamine™ RNAiMAX transfection reagent. Forty-eight hours after transfection, the medium was changed to glucose-free media. The samples were then collected for a microarray gene expression study after overnight incubation. One sample for each group.