Bulk transcriptomics of pyrethroid-treated enteroendocrine cells

Here, we performed bulk RNA sequencing analysis of the enteroendocrine cell line, STC-1 cells, following treatment with the pyrethroid pesticide, deltamethrin, as described in the associated manuscript: https://doi.org/10.1101/2024.12.14.628386. Performed through Novogene Corporation, Inc; the included "STC1_Deltamethrin.zip" file contains both a "Results" and "Report" folder. The Results folder contains an organized .html file, with detailed methods and graphical data representations of the sequencing and analysis, as performed. The Report folder contains the entirety of the analyses performed, with folders and ReadMe files for each analysis. An additional .zip file contains the aligned .bam files and their associated index files ("BamAlignment"). While a final .zip file contains the relevant fastq files for the reference genome and gene annotation ("ReferenceGenome"). A metadata file is included for sample identifications, treatment groups, and the unique IDs in the sequencing run. Raw fastq sequences are available in the NIH SRA database, under BioProject accession #: PRJNA1218260 Additional source data used in figure generation and manufacturer's protocols are available at: 10.5281/zenodo.14803680本研究按照相关论文所述方法，对经拟除虫菊酯类农药溴氰菊酯（deltamethrin）处理后的肠内分泌细胞系STC-1细胞开展了批量RNA测序（bulk RNA sequencing）分析，相关论文链接：https://doi.org/10.1101/2024.12.14.628386。 本次测序分析由诺禾致源（Novogene Corporation, Inc）完成。本次发布的"STC1_Deltamethrin.zip"压缩包包含"Results"与"Report"两个文件夹：其中Results文件夹内包含一份结构化HTML文件，详细记载了本次测序与分析的实验方法及可视化数据结果；Report文件夹则涵盖了全部已完成的分析内容，包含对应各分析模块的子文件夹与ReadMe文件。 另有一个压缩包包含比对后的BAM文件及其配套索引文件（命名为"BamAlignment"）；最后一个压缩包则包含参考基因组与基因注释所需的相关FASTQ（fastq）文件（命名为"ReferenceGenome"）。 数据集附带一份元数据文件，用于标注样本识别信息、处理分组及测序流程中的唯一识别ID。 原始FASTQ序列数据可在美国国立卫生研究院（NIH）序列读取档案（SRA）数据库中获取，对应的BioProject收录号为：PRJNA1218260。 用于图表生成的额外源数据及厂商实验操作手册可在以下链接获取：10.5281/zenodo.14803680