The B. subtilis replicative polymerases bind the sliding clamp with different strengths to tune their activity in DNA replication

This repository contains four zipped folders with data and code related to the manuscript entitled "The B. subtilis replicative polymerases bind the sliding clamp with different strengths to tune DNA replication." The folders contain: Figure data: plotted data organized by manuscript figure number (as MATLAB workspaces, .mat files). Functions: MATLAB functions needed to 1) integrate MicrobeTracker cell segmentation analysis with u-track point source detection and tracking analysis and 2) perform subsequent processing of u-track analysis results (as MATLAB scripts, .m files) Scripts: MATLAB scripts needed to perform different types of data analysis (as MATLAB scripts, .m files). These scripts are organized by type of analysis (radial distribution function analysis, diffusion coefficient analysis, etc). Sequence data: clamp-binding motif (CBM) sequences from C-family polymerases in Firmicutes species, grouped into PolC, DnaE1, and DnaE3 subtypes (as CSV files). Sequences are taken from the "representative polymerases" dataset in Timinskas, et al. Nucleic Acids Res. 2014, 42, 1393.本仓库包含四个压缩文件夹，内附与题为《枯草芽孢杆菌（Bacillus subtilis）复制型聚合酶以差异化结合强度结合滑动夹调控DNA复制》的手稿相关的数据及代码。各文件夹内容如下： 1. 图像数据：按手稿插图编号整理的绘图数据，格式为MATLAB工作区文件（.mat）。 2. 功能函数：用于完成两项任务的MATLAB函数：① 整合MicrobeTracker细胞分割分析与u-track点源检测及追踪分析流程；② 对u-track分析结果开展后续处理，文件格式为MATLAB脚本（.m）。 3. 分析脚本：用于执行各类数据分析的MATLAB脚本文件（.m），按分析类型分类整理，涵盖径向分布函数分析、扩散系数分析等方向。 4. 序列数据：夹结合基序（clamp-binding motif, CBM）序列，取自厚壁菌门（Firmicutes）物种的C家族聚合酶，分为PolC、DnaE1及DnaE3三个亚型，格式为CSV文件。序列提取自Timinskas等人2014年发表于《核酸研究》（Nucleic Acids Res.）的“代表性聚合酶”数据集，对应期刊卷42，页码1393。