Taurine depletion impairs cardiac function and affects tolerance to hypoxia and high temperatures in brook char (Salvelinus fontinalis)

Physiological and environmental stressors can cause osmotic stress in fish hearts, leading to a reduction in intracellular taurine concentration. Taurine is a beta amino acid known to regulate cardiac function in other animal models but its role in fish has not been fully elucidated. We generated a model of cardiac taurine-deficiency (TD) by feeding brook char (Salvelinus fontinalis) a diet enriched in β-alanine, which inhibits cardiomyocyte taurine uptake. Cardiac taurine levels were reduced by 21%, and stress-induced changes in normal taurine handling were observed in TD brook char. Responses to exhaustive exercise and acute thermal and hypoxia tolerance were then assessed using a combination of in vivo, in vitro, and biochemical approaches. Critical thermal maximum was higher in TD brook char despite significant reductions in maximum heart rate. In vivo, TD brook char exhibited a lower resting heart rate, blunted hypoxic bradycardia, and a severe reduction in time to loss of equilibr..., The dataset was collected and processed according to the methods described in the associated manuscript doi:10.1242/jeb.245092, Microsoft Excel or open source equivalent.

生理与环境胁迫因子可诱导鱼类心脏产生渗透胁迫，进而降低细胞内牛磺酸（taurine）浓度。牛磺酸是一种β-氨基酸，在其他动物模型中已被证实可调控心脏功能，但其在鱼类中的生理作用尚未完全阐明。本研究通过给溪红点鲑（Salvelinus fontinalis）饲喂富含β-丙氨酸（β-alanine）的饲料（β-丙氨酸可抑制心肌细胞（cardiomyocyte）牛磺酸摄取），成功构建了心脏牛磺酸缺乏症（TD）模型。该模型的心脏牛磺酸水平降低了21%，且在TD型溪红点鲑中观察到胁迫诱导的正常牛磺酸代谢调控异常。随后，我们结合体内（in vivo）、体外（in vitro）及生化实验方法，评估了受试对象对力竭运动的响应以及急性热耐受与低氧耐受能力。尽管最大心率显著降低，但TD型溪红点鲑的临界热最大值更高。体内实验显示，TD型溪红点鲑表现出更低的静息心率、低氧性心动过缓反应减弱，以及平衡丧失时间显著缩短……本数据集按照关联论文（DOI:10.1242/jeb.245092）中描述的方法进行采集与处理，可使用Microsoft Excel或开源替代软件进行相关操作。