Direct observation of common cryoprotectant permeation into rice (Oryza sativa) callus by CARS microscopy

The data and code associated with the publication "Direct observation of common cryoprotectant permeation into rice (Oryza sativa) callus by CARS microscopy" published in Cell Press. The original TIFF videos presented in the brightfield data are available upon request to from N.E.L. (lead contact), however .avi files are provided here. All .avi files are jpeg's compressed at 7 fps. https://doi.org/10.1016/j.xcrp.2023.101469 Brightfield data were collected on a confocal Olympus microscope and show live rice callus cells exposed to a variety of cryoprotectants (indicated by file names). CARS data are coherent anti-Stokes Raman scattering images of live rice callus cells exposed to a variety of deuterated cryoprotectants (indicated by file names). The R code used to analyze each data set is also provided. For a full description of the experiment and how the data were interpreted, see DOI

本数据集关联发表于细胞出版社（Cell Press）的学术论文《通过相干反斯托克斯拉曼散射（CARS）显微镜直接观察常见冷冻保护剂渗入水稻（Oryza sativa）愈伤组织》。原文明场数据中的TIFF视频需通过联系通讯作者N.E.L.申请获取，但本数据集已提供全部.avi格式文件，所有.avi文件均为7 fps帧率下的JPEG压缩格式。相关DOI链接：https://doi.org/10.1016/j.xcrp.2023.101469。明场数据采集自奥林巴斯共聚焦显微镜，展示了暴露于多种冷冻保护剂（具体种类由文件名标注）的活水稻愈伤组织细胞。CARS数据为经多种氘化冷冻保护剂处理的活水稻愈伤组织细胞的相干反斯托克斯拉曼散射成像结果，对应处理试剂由文件名标注。本数据集还附带了用于分析各数据集的R代码。如需了解实验完整流程及数据解读方式，请参阅上述DOI链接。