Cav3.2+/+ Mouse #410 CA1 EEG recording

A Ca_v3.2^+/+ mouse (internal #410) was implanted with an intrahippocampal electrode for electrohippocampal CA1 EEG recordings. The differential electrode of the TA10ETA-F20 transmitter (Data Science International, DSI, USA, technical specifications: weight 3.9 g, volume 1.9 cc, input voltage range ± 2.5 mV, channel bandwidth (B) 1–200 Hz, nominal sampling rate (f) 1000 Hz (f = 5 B), temperature operating range 34–41 °C) was positioned at the following stereotaxic coordinates: (+)-lead, caudal − 2 mm, lateral of bregma 1.5 mm (right hemisphere), and dorsoventral (depth) 1.5 mm. The epidural reference electrode was positioned on the surface of the cerebellar cortex at the following stereotaxic coordinates: (−)-lead, bregma − 6 mm and lateral of bregma 1 mm (right<br> hemisphere). The deep tungsten electrodes (FHC, USA) are encapsuled with epoxylite with an<br> impedance of 50–100 kΩ (measured at 1000 Hz) and a shank diameter of 250 μm. For further details on the Ca_v3.2 mouse model, animal experimentation approval, anaesthesia, transmitter insertion, stereotaxic electrode implantation, postoperative pain management and recovery, please refer to Arshaad MI et al., 2021. EEG data were exported to NeuroScore 3.2.9306-1 (Data Sciences International, DSI, USA). As the NeuroScore file format does not allow free access to the data, we have exported the raw EEG data as asci.-files, so that they can easily be imported in secondary analysis software. Importantly, the exported EEG data represent raw EEG data. No filtering, no artefact detection and/or artefact removal have been carried out. Thus, it is the users responsibility to adequately pretreat the data before further processing and analysis. As described in Arshaad MI et al. (2021), filtering, artefact detection and removal was carried out using NeuroScore before further analysis. The EEG data are presented here as a zip-folder for size reasons. Four EEG recordings are exported: R1 (a first 24h long-term EEG recording 10 days post transmitter implantation), R2 (a second 24h long-term recording 17 days post transmitter implantation), U1 (a 6h EEG recording following the first urethane injection (800 mg/kg i.p.) at day 18 post transmitter implantation), U2 (a 6h EEG recording after a second urethane injection (800 mg/kg i.p.) at day 25 post transmitter implantation). In addition, relative activity values are exported for all four recordings. For details on the recording procedure, please refer to Arshaad MI et al. (2021).本研究选取1只内部编号为#410的Ca_v3.2^+/+小鼠，为其植入海马内电极以开展海马CA1区脑电图（Electroencephalogram, EEG）记录。本次实验使用的TA10ETA-F20型发射器的差分电极（美国Data Science International公司，简称DSI，技术参数：重量3.9 g，体积1.9 cc，输入电压范围±2.5 mV，通道带宽（B）1–200 Hz，标称采样率（f）1000 Hz（f = 5 B），工作温度范围34–41 ℃）被定位至以下立体定位坐标：（+）极：相对于前囟（bregma）尾侧−2 mm、右侧半球旁开1.5 mm，背腹向（深度）1.5 mm。硬膜外参考电极置于小脑皮质表面，其立体定位坐标为：（-）极：相对于前囟尾侧−6 mm、右侧半球旁开1 mm。本实验使用的深层钨电极（美国FHC公司）采用环氧基树脂（epoxylite）封装，阻抗为50–100 kΩ（1000 Hz下测得），电极柄直径为250 μm。 关于Ca_v3.2小鼠模型、动物实验伦理审批、麻醉操作、发射器植入流程、立体定位电极植入、术后镇痛与恢复的更多细节，请参见Arshaad MI等人2021年发表的研究。 脑电数据首先被导出至NeuroScore 3.2.9306-1软件（美国Data Science International公司，简称DSI）。由于NeuroScore文件格式无法直接开放数据访问，我们将原始脑电数据导出为asci.-文件，以便用户可轻松将其导入至二次分析软件中。重要提示：本数据集导出的脑电数据均为原始脑电数据，未经过滤波、伪迹检测和/或伪迹去除处理。因此，用户需在开展后续处理与分析前，对数据进行恰当的预处理。如Arshaad MI等人（2021）所述，在进行后续分析前，已使用NeuroScore软件完成滤波、伪迹检测与去除操作。 考虑到文件大小，本数据集的脑电数据以zip压缩包形式提供。本次共导出4组脑电记录：R1为发射器植入后第10天的首次24小时长期脑电记录；R2为发射器植入后第17天的第二次24小时长期脑电记录；U1为发射器植入后第18天首次腹腔注射（intraperitoneal, i.p.）氨基甲酸乙酯（urethane，800 mg/kg）后的6小时脑电记录；U2为发射器植入后第25天第二次腹腔注射氨基甲酸乙酯（urethane，800 mg/kg）后的6小时脑电记录。此外，本数据集还为4组脑电记录均导出了相对活动度数值。关于记录流程的更多细节，请参见Arshaad MI等人（2021年）的研究。