Towards 3D structure determination and dynamics of biological and non-biological samples using TR-SSX methods

This BAG proposal aims to conduct room temperature time-resolved serial crystallography experiments at the new ID29 beamline at ESRF. We intend to collect full data sets on microcrystals of two medically relevant proteins, the penicillin binding protein 2a (PBP2a) of methicillin resistant staphyloccocus aureus (MRSA) and the human the NAP(P)H:quinone oxidoreductase 1 (NQO1) protein, with their respective ligands. As well, we aim at conducting pump-probe time-resolved crystallography experiments on microcrystals of metal organic frameworks (MOFs). The good quality of all crystalline samples has been proved either at ESRF beamlines ID30-A1 and ID30-A3, or at other synchrotron beamlines. At ID29, they offer a variety of sample delivery devices including high viscosity injectors, tape drives, fixed target supports, and microfluidic devices, that will be employed in our experiments depending on sample needs.

本BAG申请旨在欧洲同步辐射光源（ESRF，European Synchrotron Radiation Facility）新建的ID29光束线开展室温时间分辨串行晶体学实验。我们计划针对两种医学相关蛋白的微晶体采集完整数据集：分别是耐甲氧西林金黄色葡萄球菌（MRSA，Methicillin-resistant Staphylococcus aureus）的青霉素结合蛋白2a（PBP2a，Penicillin-binding protein 2a），以及人类NAP(P)H:醌氧化还原酶1（NQO1，NAP(P)H:quinone oxidoreductase 1），并将其与各自配体共同开展实验。此外，我们还计划针对金属有机框架（MOFs，Metal-Organic Frameworks）的微晶体开展泵浦-探测型时间分辨晶体学实验。所有晶体样品的优异品质均已在ESRF的ID30-A1、ID30-A3光束线或其他同步辐射光束线上得到验证。ID29光束线可提供多种样品递送装置，包括高黏度进样器、胶带传送装置、固定靶支架以及微流控设备，我们将根据样品需求在实验中选用对应的装置。