AcM-UBE2M transfers NEDD8 to CRL3 E3 ubiquitin ligase complex

UBE2M transfers NEDD8 to lysine 712 of CUL3 in the E3 ligase complex (Hori et al, 1999; Duda et al, 2008). Neddylation increases the ubiquitin ligase activity of the E3 complex towards its targets, and prevents binding of the CUL3 complex with the CAND1 inhibitor (Hori et al, 1999; Duda et al, 2008). CRL3 ubiquitin ligase complexes target substrates through a BTB substrate recognition protein, and regulate processes such as development and stress response, as well as viral replication (reviewed in Genschik et al, 2013; Mahon et al, 2014).

UBE2M将NEDD8转移至E3连接酶复合物中CUL3的赖氨酸712位点（Hori等，1999；Duda等，2008）。N端乙酰化作用可增强E3复合物对其靶点的泛素连接酶活性，并防止CUL3复合物与CAND1抑制剂结合（Hori等，1999；Duda等，2008）。CRL3泛素连接酶复合物通过BTB底物识别蛋白靶向底物，调控发育、应激反应以及病毒复制等过程（参见Genschik等，2013；Mahon等，2014年综述）。