Coral physiology parameters acquired during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.

Two common reef-building corals, Montipora capitata and Pocillopora acuta, were collected from six sites in Kāne'ohe Bay, O'ahu, Hawai'i. Fragments were allowed to acclimate in experimental tanks for two weeks prior to exposure to one of the following four treatments: Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC), and High Temperature High pCO2 (HTHC). The treatment period lasted for a two month period, starting on September 22nd, 2018 and lasting through November 17th, 2018. Following the stress period, coral fragments were exposed to a two-month recovery period in ambient conditions.

Throughout the entire four-month experiment, fragments were randomly sampled from each tank and treatment for the following physiology parameters: gross photosynthesis, respiration, net photosynthesis (gross photosynthesis - respiration), photosynthesis:respiration ratio, chlorophyll concentration (pigment a and c2), symbiont and host tissue biomass, symbiont:host tissue biomass ratio, host soluble protein, host total antioxidant capacity, and endosymbiont density. Net photosynthesis, chlorophyll concentration, and symbiont tissue biomass were normalized to host surface area (cm-2) and endosymbiont density (cell-1).

本研究采集了夏威夷瓦胡岛卡内奥赫湾（Kāne'ohe Bay）6个采样点的两种常见造礁珊瑚：卷叶微孔珊瑚（Montipora capitata）与尖枝列孔珊瑚（Pocillopora acuta）。将珊瑚断枝置于实验水箱中驯化适应两周，随后分别接受以下4种处理：常温常二氧化碳分压（Ambient Temperature Ambient pCO₂，ATAC）、常温高二氧化碳分压（Ambient Temperature High pCO₂，ATHC）、高温常二氧化碳分压（High Temperature Ambient pCO₂，HTAC）以及高温高二氧化碳分压（High Temperature High pCO₂，HTHC）。处理周期时长为2个月，始于2018年9月22日，至2018年11月17日结束。胁迫处理阶段结束后，将珊瑚断枝置于自然环境条件下开展为期2个月的恢复培养。

在整个为期4个月的实验周期内，研究人员从各水箱及各处理组中随机采集珊瑚断枝，用于测定以下生理参数：总光合速率、呼吸速率、净光合速率（总光合速率-呼吸速率）、光合/呼吸速率比、叶绿素浓度（叶绿素a与c₂）、共生体与宿主组织生物量、共生体/宿主组织生物量比、宿主可溶性蛋白含量、宿主总抗氧化能力以及内共生体密度。其中，净光合速率、叶绿素浓度及共生体组织生物量均以宿主表面积（cm⁻²）为基准进行标准化校正，内共生体密度则以每细胞（cell⁻¹）为单位完成标准化处理。