Cloning, characterization, antibacterial activity and expression of hamp in pond loach (Misgurnus anguillicaudatus) after bacterial challenge with Aeromonas hydrophila

Supplementary Fig. 1 The detailed primer reports of hamp 1a and 1b which were used to determine the expression pattern in qRT-PCR Supplementary Fig. 2 Phylogenic tree of hepcidin amino acid sequences constructed using the neighbor-joining method. Supplementary Fig. 3 The plot shows the posterior probabilities of inside/outside/transmembrane helices. Supplementary Fig. 4 (A) Three-dimensional structures of M. anguillicaudatus Hepcidin protein obtained using SWISS-MODEL Supplementary Table 1. GenBank accession numbers for hepcidin homologs Supplementary Table 2. Assessment of predicted three-dimensional structure of hepcidin protein by different validation methods. Supplementary Table 3. Predicted protein interactions for the M. anguillicaudatus hepcidin 1 inferred using STRING algorithm Supplementary Table 4. Basal mRNA expression of the two paralogs in different tissues relative to β-actin Supplementary Table 5. mRNA expression of the two paralogs in different tissues after the challenge with A. hydrophila

补充图1 用于通过实时定量逆转录PCR（qRT-PCR）确定基因表达模式的hamp 1a与hamp 1b的详细引物信息
补充图2 采用邻接法构建的铁调素（hepcidin）氨基酸序列系统发育树
补充图3 该图展示了膜内、膜外与跨膜螺旋的后验概率
补充图4(A) 采用SWISS-MODEL工具预测获得的泥鳅（Misgurnus anguillicaudatus）铁调素蛋白三维结构
补充表1 铁调素同源基因的GenBank登录号
补充表2 采用多种验证方法对铁调素蛋白预测三维结构的评估结果
补充表3 采用STRING算法预测得到的泥鳅铁调素1的潜在蛋白质相互作用关系
补充表4 以β-肌动蛋白（β-actin）为内参基因，两个旁系同源基因在不同组织中的基础mRNA表达水平
补充表5 嗜水气单胞菌（Aeromonas hydrophila）攻毒处理后，两个旁系同源基因在不同组织中的mRNA表达水平