Additional SMLM Data from "Reinforced optical cage systems enable drift-free single-molecule localization microscopy"

This dataset contains additional data for the paper titled 'Reinforced optical cage systems enable drift-free single-molecule localization microscopy' that has been generated to address reviewer comments. The dataset has the following: Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data. Used for axial drift and lateral drift quantification. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a commercial microscope. .tif files for images, .csv files for localization data Two STORM image series' of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 acquired with a homebuilt microscope and localization data. .tif files for images, .csv files for localization data Two STORM image series' of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 acquired with a commercial microscope and localization data. .tif files for images, .csv files for localization data. Two STORM image series' of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a homebuilt microscope and localization data. .tif files for images, .csv files for localization data. Two STORM image series' of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a commercial microscope and localization data. .tif files for images, .csv files for localization data.本数据集为响应审稿人意见而生成，配套于论文《强化光学笼式系统实现无漂移单分子定位显微成像》（Reinforced optical cage systems enable drift-free single-molecule localization microscopy），包含该论文的补充实验数据，具体内容如下： 1. 自制显微镜采集的5组荧光微球图像序列及其每帧亚像素定位结果：图像文件格式为.tif，定位数据文件格式为.csv，可用于轴向漂移与横向漂移的量化分析。 2. 商用显微镜采集的5组荧光微球图像序列及其每帧亚像素定位结果：图像文件格式为.tif，定位数据文件格式为.csv。 3. 自制显微镜采集的2组α微管蛋白经Alexa Fluor 647偶联抗体免疫标记的HeLa细胞STORM（随机光学重构显微术，stochastic optical reconstruction microscopy）图像序列及其定位数据：图像文件格式为.tif，定位数据文件格式为.csv。 4. 商用显微镜采集的2组α微管蛋白经Alexa Fluor 647偶联抗体免疫标记的HeLa细胞STORM图像序列及其定位数据：图像文件格式为.tif，定位数据文件格式为.csv。 5. 自制显微镜采集的2组Nup96-SNAP-AF647标记的U2OS细胞STORM图像序列及其定位数据：图像文件格式为.tif，定位数据文件格式为.csv。 6. 商用显微镜采集的2组Nup96-SNAP-AF647标记的U2OS细胞STORM图像序列及其定位数据：图像文件格式为.tif，定位数据文件格式为.csv。