Transcriptional profiling of melanoma-associated lymphatic endothelial cells (LECs)

Objective was to uncover the transcriptional changes occuring in melanoma-associated LECs relative to LECs of naïve skin. To investigate the transcriptional changes in LECs during tumor development, RNA was extracted from LECs sorted from naïve skin and BPC (Tyr::Cre-ER, BRafV600E) murine melanomas and bulk RNA-seq performed. Naïve skin LECs were collected from 3 mice and tumor-associated LECs collected from 4 mice. Overall design: Comparative gene expression profiling analysis of RNA-seq data from LECs isolated from murine naïve skin or BPC melanomas.