PARAGON 1 - KM2112 - Total Organic Carbon Timecourse Incubations

This dataset contains measurements of total organic carbon concentrations (TOC) collected during the PARAGON 1 expedition (KM2112) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Samples for measurements of TOC concentrations were collected at multiple time points for each experimental biological replicate by aliquoting 40 mL of whole seawater from polycarbonate incubation bottles into pre-combusted borosilicate vials. Samples were acidified with 27 µL of 12N HCl (Optima grade, Fisher), capped with Teflon-lined silicone septa lids, and stored in the dark at room temperature until analysis on shore. Total organic carbon concentrations were determined by high temperature combustion on a modified Shimadzu TOC analyzer according to Carlson et al. (2010). Timestamp is in UTC. Version 2 corrects formatting errors in the timestamp.本数据集收录了北太平洋副热带环流（North Pacific Subtropical Gyre）PARAGON 1航次（KM2112）期间采集的总有机碳（total organic carbon, TOC）浓度测量数据。该数据集的测量结果源自时间序列培养实验：实验采用痕量金属洁净技术采集150米深度的全海水作为初始样本，并通过添加不同梯度的有机碳、铁和/或氮进行改性处理。针对每个实验生物学重复，研究人员在多个时间点采集TOC浓度测量样本：将聚碳酸酯培养瓶中的全海水分装40mL至预先灼烧过的硼硅酸盐小瓶中。样本中加入27微升12N盐酸（Optima级，Fisher品牌），使用特氟龙衬垫硅胶隔垫瓶盖密封后，于室温避光储存，直至岸基分析。总有机碳浓度采用高温燃烧法，通过改装后的岛津（Shimadzu）TOC分析仪进行测定，方法参照Carlson等（2010）的研究。时间戳采用协调世界时（UTC）。V2版本修正了时间戳的格式错误。