Whole genome sequencing of Cronobacter sakazakii MK_10

Cronobacter sakazakii MK_10 was isolated from dried oregano and sequenced using a hybrid strategy combining Oxford Nanopore Technologies (ONT) and Illumina platforms. For ONT sequencing, genomic DNA was mechanically fragmented using BD Micro-Fine Plus needles, and libraries were prepared with the Ligation Sequencing Kit and Native Barcoding Kit 96 V14 (SQK-NBD114.96; Oxford Nanopore Technologies). Library quality was evaluated using an Agilent TapeStation 2200 system with Genomic DNA ScreenTape, and sequencing was carried out on a MinION device equipped with an R10.4.1 flow cell.For Illumina sequencing, DNA was fragmented with a Covaris S220 ultrasonicator, followed by library preparation using the KAPA HyperPrep Kit and TruSeq DNA UD Indexes. Quality control was performed on an Agilent TapeStation 2200 system with High Sensitivity D1000 reagents. Libraries were quantified by qPCR using the KAPA Library Quantification Kit, and fragment size distribution was assessed prior to sequencing. Sequencing was conducted on an Illumina NovaSeq 6000 instrument in paired-end mode using an S1 Reagent Kit v1.5.