Mitonuclear interactions and early-life diet shape adult nutritional behaviour

Mitochondrial function relies on close coordination between the mitochondrial and nuclear genomes. Disruption to this coordination—via mitonuclear mismatch—can impair metabolic efficiency, particularly under energetically demanding conditions such as during development. The nutritional environment further modulates mitochondrial demands, suggesting that mitonuclear genotype and diet may interact to shape life-history traits and behaviour. Here, we investigate how early-life diet and mitonuclear genotype jointly influence development time, adult body size, and nutritional preference in Drosophila melanogaster. Using a full-factorial panel of putatively matched and mismatched combinations (cybrids) of mitonuclear genotype derived from natural Australian populations, we reared flies on diets varying in their ratio of macronutrients and assessed how this influenced larval development and subsequent adult diet preference. Developmental rate was significantly influenced by mitonuclear coevolu..., , # Mitonuclear interactions and early-life diet shape adult nutritional behaviour

Dataset DOI: [10.5061/dryad.98sf7m0wz](10.5061/dryad.98sf7m0wz)

## Description of the data and file structure

Two experiments were run for this project. The first is a diet choice (dietchoice.csv) and the second is a diet preference assay (devtime.csv)

### Files and variables

#### File: dietpref.csv

**Description:** 

##### Variables

* block: unit of measure where the whole experiment was replicated 
* sex: male or female fly
* nuc: nuclear genome fly harboured
* mito: mtDNA genotype of fly
* diet: diet that flies were developed on (D = high carb, B = standard, A = high protein)
* vial: vial number (within diet) that the flies developed in
* totalp: total amount of protein consumed across 3 days of experiment - *unit of measurement: ul*
* totalc: total amount of carbohydrate consumed across 3 days of experiment- *unit of measurement: ul*
* weight: dry mass of the fly following the experiment - *unit ...,

线粒体功能依赖于线粒体基因组与核基因组的紧密协同。这种协同被打破（通过核质不匹配，mitonuclear mismatch）会损害代谢效率，尤其是在发育等能量需求较高的条件下。营养环境可进一步调控线粒体需求，这提示核质基因型（mitonuclear genotype）与饮食或可相互作用，共同塑造生命史特征与行为模式。本研究旨在探究早期饮食与核质基因型如何共同影响黑腹果蝇（Drosophila melanogaster）的发育时长、成虫体型及营养偏好。我们采用源自澳大利亚自然种群的、包含推定匹配与不匹配核质基因型组合（胞质杂种，cybrids）的全因子实验体系，在宏营养素比例不同的培养基上饲养果蝇，并评估其对幼虫发育及后续成虫营养偏好的影响。发育速率显著受核质共进化……，# 核质互作与早期饮食塑造成虫营养行为
数据集DOI：[10.5061/dryad.98sf7m0wz](10.5061/dryad.98sf7m0wz)

## 数据与文件结构描述
本项目开展了两项实验：其一为饮食选择实验（dietchoice.csv），其二为饮食偏好测定实验（devtime.csv）

### 文件与变量
#### 文件：dietpref.csv
**描述：**

##### 变量
* block：整个实验重复的测量单元
* sex：雄性或雌性果蝇
* nuc：果蝇所携带的核基因组
* mito：果蝇的线粒体DNA（mtDNA）基因型
* diet：果蝇发育所处的培养基（D代表高碳水化合物，B代表标准培养基，A代表高蛋白）
* vial：果蝇发育所在的培养管编号（同一培养基组内）
* totalp：实验持续3天期间的总蛋白质消耗量——*测量单位：微升（ul）*
* totalc：实验持续3天期间的总碳水化合物消耗量——*测量单位：微升（ul）*
* weight：实验结束后果蝇的干重——*测量单位：……