Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments

Data underlying the figures in the publication “Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments”, published in <em>Adv. Mater</em>., <strong>2020</strong>, 32, 2004804. https://onlinelibrary.wiley.com/doi/full/10.1002/adma.202004804 Table of contents: <strong>1. Figure 2</strong>; Zip file containing the data for <em>Figure 2</em>. It contains 5 files with the corresponding data: <strong> 1.1 Figure 2a-b</strong>: Microscopy images and a video of the emulsions flowing in the main channel of the microfluidic chip, and data for <em>Figure 2b</em> containing the numerical data for the histograms and their corresponding regressed normal distributions out of 2361 measured droplets. <strong> 1.2 Figure 2c</strong>: Dataset for <em>Figure 2c</em>, containing the numerical data for the inner and outer diameter variation as a function of the outer phase flow rate. <strong> 1.3 Figure 2d</strong>: Dataset for <em>Figure 2d</em>, containing the numerical data for the inner and outer diameter variation as a function of the flow rate ratio. <strong> 1.4 Figure 2f</strong>: Dataset for <em>Figure 2f</em>, containing the numerical data for the histogram and its regressed normal distribution and the corresponding image. <strong> 1.5 Figure 2g-h-i</strong>: Dataset for <em>Figure 2g, 2h</em> and <em>2i</em>, containing the CLSM image of GUVs loaded with carboxyfluorescein at the beginning of the permeability experiment and the numerical values for the fluorescence studies (<em>Figures 2h</em> and <em>2i</em>). <strong>2. Figure 4</strong>; Zip file containing the data for <em>Figure 4h-q</em>. It contains 10 files with the numerical data for the fluorescence study graphs showing the change in intensity over time of fluorescein (<em>Figures 4h-l</em>) and resorufin (<em>Figures 4</em>m-q).本数据集支撑发表于《先进材料（Adv. Mater.）》2020年第32卷，文章编号为2004804的论文《双乳液模板化细胞尺度区室生化通路研究的组合策略》，论文链接：https://onlinelibrary.wiley.com/doi/full/10.1002/adma.202004804 数据集目录： 1. 图2数据集：包含对应图2数据的压缩包，内有5个相关数据文件： 1.1 图2a-b：微流控芯片主通道内乳液流动的显微图像与视频，以及图2b的数值数据，涵盖对2361个被测液滴统计得到的直方图及其拟合正态分布的相关数据。 1.2 图2c：对应图2c的数据集，包含外相流速与内外直径变化关系的数值数据。 1.3 图2d：对应图2d的数据集，包含流速比与内外直径变化关系的数值数据。 1.4 图2f：对应图2f的数据集，包含直方图及其拟合正态分布的数值数据与对应图像。 1.5 图2g-h-i：对应图2g、2h与2i的数据集，包含渗透实验初始时刻加载羧基荧光素的巨型单层囊泡（giant unilamellar vesicles, GUVs）的共聚焦激光扫描显微镜（confocal laser scanning microscopy, CLSM）图像，以及对应图2h、2i的荧光实验数值结果。 2. 图4数据集：包含对应图4h-q数据的压缩包，内有10个数值数据文件，用于绘制荧光研究图表，展示荧光素（对应图4h-l）与试卤灵（对应图4m-q）的强度随时间变化情况。