Supporting isotopic data for: Differential utilization of submerged leaf litter by microbial biofilms and macroinvertebrates in a large dryland river

In river food webs, the energetic coupling of nutrient-rich ‘fast’ autochthonous (algal) and recalcitrant ‘slow’ allochthonous (terrestrial) resources may confer stability. Additionally, microbial biofilms may enhance the nutritional quality of allochthonous resources for macroinvertebrate consumers, potentially facilitating trophic linkages within slow energy channels. We used 16S and 18S rRNA gene sequencing and essential amino acid (AAESS) carbon isotope (δ13C) analysis to characterize microbial biofilms and quantify proportional contributions of AAESS derived from algae, terrestrial plants, and microbes (Archaea, Bacteria, fungi) to macroinvertebrates across a series of mesocosm and in situ leaf pack experiments in the middle Rio Grande of central New Mexico, USA. In our 12-week mesocosm experiment, we found that leaves of native Rio Grande Cottonwood trees (Populus deltoides wislizeni) contributed greater mean estimated proportions of AAESS (0.41–0.81) to Chironomidae larvae t..., , # Supporting isotopic data for: Differential utilization of submerged leaf litter by microbial biofilms and macroinvertebrates in a large dryland river

**Alexi C. Besser**

Dataset DOI: [10.5061/dryad.2fqz61326](10.5061/dryad.2fqz61326)

## Description of the data and file structure

Stable carbon isotope values of individual amino acids were measured via gas chromatography-combustion-isotope ratio mass spectrometry after derivatization to N-trifluoroacetic acid isopropyl esters at the University of New Mexico Center for Stable Isotopes. Stable carbon isotope values are expressed in delta notation (delta = [(Rsample – Rreference)/Rreference], where R = the ratio of 13C to 12C) and reported in parts per thousand (i.e., per mil). The international reference standard for stable carbon isotope analysis is Vienna Pee Dee Belemnite (VPDB). Additional information on sample collection, preparation, analysis, and data correction can be found in the accompanying manuscript and supporting informa...,

在河流食物网中，富营养的‘快速’本土源性（藻类）资源与难降解的‘慢速’外来源性（陆地）资源之间的能量耦合，或许能够维持系统稳定性。
此外，微生物生物膜可提升外来源性资源对大型无脊椎动物消费者的营养品质，进而可能促进慢速能量通道内的营养联系。
本研究依托美国新墨西哥州中部格兰德河中游区域的一系列中宇宙模拟实验与原位叶包实验，采用16S与18S rRNA基因测序技术、必需氨基酸（essential amino acid, AAESS）碳同位素（δ¹³C）分析方法，对微生物生物膜进行表征，并量化藻类、陆地植物与微生物（古菌、细菌、真菌）来源的AAESS在大型无脊椎动物体内的比例贡献。
在为期12周的中宇宙模拟实验中，我们发现本土格兰德河三角叶杨（Populus deltoides wislizeni）的叶片为摇蚊科幼虫提供的AAESS平均估计占比更高（0.41~0.81），[原文未完结]

# 支撑同位素数据：大型旱地河流中微生物生物膜与大型无脊椎动物对浸没性落叶凋落物的差异化利用

**亚历克西·C·贝瑟**
数据集DOI：[10.5061/dryad.2fqz61326](10.5061/dryad.2fqz61326)

## 数据与文件结构说明
单个氨基酸的稳定碳同位素值在新墨西哥大学稳定同位素中心完成测定：样品先衍生化为N-三氟乙酸异丙酯，随后通过气相色谱-燃烧-同位素比率质谱法进行检测。
稳定碳同位素值以δ符号表示（δ = [(R样本 – R参比)/R参比]，其中R为¹³C与¹²C的比值），单位为千分比（即per mil）。
稳定碳同位素分析的国际参考标准为维也纳佩德迪贝莱姆尼特（Vienna Pee Dee Belemnite, VPDB）。
关于样品采集、前处理、分析与数据校正的更多细节，可参见随附的研究论文与补充材料[原文未完结]