Reproducible scRNA-seq PBMC Workflow: Reference, Toy Demonstration Data, and Analysis Outputs

This record contains a fully reproducible, containerized single-cell RNA-seq workflow for PBMC data analysis. The workflow implements: FASTQ quality control STARsolo alignment Seurat-based QC, normalization, clustering, and annotation Pseudobulk differential expression and TOST equivalence testing Cell-type–specific co-expression network construction Version 1 provides a lightweight demonstration bundle including: A chromosome 1 (chr1) GRCh38 mini-reference A pre-built STAR index for chr1 A reduced FASTQ subset (~100k reads) for rapid execution This demonstration dataset is designed to run in 5–10 minutes on a standard workstation and illustrates full pipeline functionality without requiring large memory resources. Future versions of this record will include the full GRCh38 reference, complete STAR index, and full-scale analysis outputs.本数据集包含一套可完全复现、容器化的外周血单个核细胞（Peripheral Blood Mononuclear Cell, PBMC）数据分析单细胞RNA测序（single-cell RNA-seq）工作流。 该工作流涵盖以下分析流程： - FASTQ质量控制 - STARsolo序列比对 - 基于Seurat的质量控制、标准化、聚类与细胞注释 - 伪bulk差异表达分析及TOST等效性检验 - 细胞类型特异性共表达网络构建 第1版提供轻量化演示套件，包含： - 针对1号染色体（chr1）的GRCh38迷你参考基因组 - 适配chr1的预构建STAR索引 - 用于快速运行的缩减型FASTQ子集（约10万条reads） 该演示数据集可在标准工作站上于5至10分钟内完成运行，无需占用大量内存即可完整展示该流水线的全部功能。 本数据集后续版本将包含完整的GRCh38参考基因组、完整STAR索引及全规模分析输出结果。