Cape Darnley Early-Autumn Phytoplankton Bloom, March 2012

These data relate to a large-scale early-autumn phytoplankton bloom that occurred off Cape Darnley, East Antarctica, in March 2012. The bloom was detected by Dr Jan Lieser (Antarctic Climate and Ecosystems Cooperative Research Centre, ACE-CRC) through MODIS satellite and was opportunistically sampled from RSV Aurora Australis using the uncontaminated seawater line. Samples were analysed for protist species and abundances using light and scanning electron microscopy, and pigment analyses were conducted using high performance liquid chromatography. Additional water samples were taken for dissolved nutrient analyses.

Specific details of the files are:

Cape Darnley Protist Counts
Samples were preserved with 1 % vol:vol Lugols iodine and stored in glass bottles in the dark at 4 degrees C. Protists were identified and counted using phase and Nomarski interference optics using Olympus IX71 and IX81 inverted microscopes at 400X to 640X magnification. Bright field optics were also used to discriminate taxa that contained chloroplasts. Protistan taxa were counted in 20 randomly chosen fields of view, except for highly abundant taxa that were counted in a subset of the field of view defined by an ocular quadrant (Whipple grid). Cell biovolumes and carbon conversion statistics were used to calculate the cell biomass of protistan taxa/groups.

Cape Darnley Fluorometer Calibration
Fluorometer measurements from the ships underway system were calibrated using chlorophyll a readings determined through high performance liquid chromatography. A linear relationship was established between fluorometer v HPLC chlorophyll a measurements at the same sites. The linear equation was then used to convert all underway fluorometry data from the voyage.

Cape Darnley Bloom HPLC Pigments CHEMTAX summary
Major phytoplankton groups at each site determined through analysis of pigments using high performance liquid chromatography and CHEMTAX. Methods were according to that of Wright et al. (2010).

Cape Darnley Bloom Nutrients
Dissolved nutrient concentrations. Samples were analysed by the Department of Primary Industries, Parks, Water and Environment, 18 St. Johns Avenue, Newtown, Tasmania 7008.

Cape Darnley Underway Data VOYAGE_04_0_201112
Raw underway data from Aurora Australis in the bloom region

Cape Darnley Underway Data Maps
Maps of the underway data in the bloom region

本数据集关联2012年3月于南极东部达恩利角周边海域发生的一次大规模初秋浮游植物水华（phytoplankton bloom）。该水华由简·利瑟博士（Jan Lieser）依托南极气候与生态系统合作研究中心（ACE-CRC），通过MODIS卫星发现，并由RSV Aurora Australis号科考船采用无污染海水采样管线完成机会性采样。研究人员采用光学显微镜与扫描电子显微镜对样本中的原生生物（protist）种类与丰度进行分析，并通过高效液相色谱法（high performance liquid chromatography, HPLC）开展色素检测；此外还采集了额外水样用于溶解态营养盐分析。

各文件具体说明如下：

Cape Darnley Protist Counts（达恩利角原生生物计数数据）
样本采用1%体积比的卢戈氏碘液固定，置于棕色玻璃瓶中于4℃暗处保存。研究人员使用奥林巴斯IX71与IX81倒置显微镜，以400倍至640倍放大倍率，通过相差与Nomarski干涉相差光学系统完成原生生物的鉴定与计数；同时辅以明场光学系统区分含有叶绿体的类群。除丰度极高的类群采用目镜四分视场（Whipple网格）划定的局部视场计数外，其余原生生物类群均在20个随机选取的视场中完成计数。通过细胞生物体积与碳转换系数计算各原生生物类群/类组的细胞生物量。

Cape Darnley Fluorometer Calibration（达恩利角荧光计校准数据）
本次对科考船走航系统获取的荧光计测量数据，以高效液相色谱法测定的叶绿素a（chlorophyll a）读数完成校准。在同一采样点位建立了荧光计测量值与HPLC叶绿素a测量值之间的线性关系，随后利用该线性方程转换本次科考的全部走航荧光法数据。

Cape Darnley Bloom HPLC Pigments CHEMTAX summary（达恩利角水华HPLC色素与CHEMTAX汇总数据）
通过高效液相色谱法与CHEMTAX软件对各点位的色素进行分析，以此确定各点位的主要浮游植物类群。分析方法参照Wright等人2010年发表的研究方案。

Cape Darnley Bloom Nutrients（达恩利角水华营养盐数据）
溶解态营养盐浓度数据。样本由塔斯马尼亚州纽敦市圣约翰大道18号的初级产业、公园、水利与环境部门完成分析。

Cape Darnley Underway Data VOYAGE_04_0_201112（达恩利角走航数据 VOYAGE_04_0_201112）
RSV Aurora Australis号科考船在水华海域获取的原始走航数据。

Cape Darnley Underway Data Maps（达恩利角走航数据地图）
水华海域走航数据相关可视化地图。