Comparing preprocessing strategies for 3D-Gene microarray data of extracellular vesicle-derived miRNAs

miRNAs derived from serum EVs of ALS patients obtained in two batches Venous blood samples were collected from 28 and 47 patients from the first and second cohorts, respectively, to isolate EVs and perform miRNA microarray analyses. The first and second cohorts had 10 common patients, for whom we evaluated data consistency and batch effects in a preprocessing pipeline comparison. If S001-S010 in batches 1 and 2 have the same number, they refer to samples obtained from the same patient.