(15)-Kraemer2026A-DS0006 – SLICE-2 – Tribolium castaneum AGOC{ATub'H2A/H2B°NB-mEmerald} #1 subline fused/deconvolved long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy – Part 5/6

The second Systematic Live Imaging Collection of Embryogenesis (SLICE-2) is a collection of sixteen isotropic 3D fluorescence live imaging datasets documenting gastrulation and early germband elongation in the red flour beetle Tribolium castaneum. Imaging was performed using light sheet fluorescence microscopy (LSFM) in combination with the transgenic AGOC{ATub’H2A/H2B°NB-mEmerald} #1 subline, which expresses mEmerald-labeled nanobodies against histone H2A/H2B under the control of the constitutively and ubiquitously active tubulin alpha 1-like protein promoter. Each embryo was recorded in a single fluorescence channel along four directions spaced 90° apart. Recordings comprised up to 51 time points at 30 min intervals, corresponding to a total imaging duration of up to 25 h. The resulting image stacks were subjected to multiview fusion to generate isotropic 3D reconstructions of the data, which are provided as two different derivatives: weighted-average fusions and fusion-deconvolutions. Each dataset comprises one recording of one embryo and was processed using multiple image-processing branches. For convenience, the data are distributed across multiple archive parts corresponding to these branches: the basic branch (B) – part 1 the weighted-average fusion (F) and fusion-deconvolution (D) branches – part 2 the preliminary processing branch (P) – part 3 (and optionally parts 4–6 for selected datasets) Entries belonging to the same dataset are clearly labeled in the entry title and cross-linked in the ‘Additional details’ section. Each entry also includes comprehensive experimental metadata in the form of a human- and machine-readable XLSX file. In addition, the first entry of each dataset contains intensity-adjusted z projection t stack montage TIFF files together with corresponding AVI files for rapid inspection of the dataset in Fiji or any standard movie player. Detailed descriptions of the experimental procedures, image-processing workflow, folder structure, and file organization are provided in the associated Data Descriptor.第二代胚胎发育系统性活体成像数据集（SLICE-2）是包含16套各向同性三维荧光活体成像数据集的集合，用于记录赤拟谷盗（Tribolium castaneum）的原肠胚形成与早期胚带延伸过程。该成像采用光片荧光显微镜（light sheet fluorescence microscopy, LSFM），结合转基因品系AGOC{ATub’H2A/H2B°NB-mEmerald}#1亚系完成：该品系在组成型泛表达的微管蛋白α1样蛋白启动子调控下，表达针对组蛋白H2A/H2B的mEmerald标记纳米抗体。 每个胚胎均在单个荧光通道中沿四个间隔90°的方向完成成像记录。采集序列包含最多51个时间点，时间间隔为30分钟，总成像时长最长可达25小时。所得图像堆栈经多视图融合处理以生成各向同性三维重建数据，提供两种不同的衍生数据格式：加权平均融合结果与融合反卷积结果。 每个数据集对应单个胚胎的一次成像记录，并通过多条图像处理分支完成处理。为便于使用，数据集按对应不同图像处理分支分为多个归档分卷： - 基础分支（B）——分卷1 - 加权平均融合（F）与融合反卷积（D）分支——分卷2 - 预处理分支（P）——分卷3（部分选中数据集可额外提供分卷4至6） 同一数据集的条目在条目标题中均有明确标注，并在"Additional details"章节中相互交叉引用。每个条目还包含可被人类与机器读取的XLSX格式实验元数据文件。此外，每个数据集的首个条目包含经强度调整的Z轴投影堆叠拼接TIFF文件，以及对应的AVI视频文件，可在Fiji或任意标准视频播放器中快速预览数据集。 实验流程、图像处理工作流、文件夹结构与文件组织的详细说明均收录于配套的数据描述文档中。