LH3 overexpression reverses profound alterations of gene transcriptional profile in cerebral vessels in hypertension-induced spontaneous intracerebral hemorrhage

To gain mechanistic insights into the protection effects of lysyl hydroxylase (LH3) on reducing the susceptibility to intracerebral hemorrhage(ICH), a whole transcriptomic analysis of cerebral vessels were performed using RNA-seq. It is noteworthy that our preliminary data demonstrated that mice did not developed ICH until day 7 after AngII plus L-NAME infusion, cerebral vessels for RNA-seq were acquired at day 5 in order to provide the mechanistic or causal evidence of a direct participatory role of LH3 to the protection effects of ICH. 15 samples. There are 3 groups: GFP-Veh group, mice were stereotactic injection of AAV-GFP and treated with saline. GFP-AL group, mice were stereotactic injection of AAV-GFP and treated with AngII plus L-NAME. LH3-AL group, mice were stereotactic injection of AAV-LH3 and treated with AngII plus L-NAME. Mice of each group were transcardially perfused with ice-cold phosphate-buffered saline, brain were collected and cerebral vessels were isolated.