RNAseq

Whole transcriptome analysis was performed on mouse splenocytes under four conditions: uninfected, infected, uninfected treated with XAV939, and infected treated with XAV939. Sequencing generated high-quality reads, which were processed and mapped to the Mus musculus genome. Differential gene expression analysis revealed significant dysregulation in inflammatory, metabolic, and redox pathways during Toxoplasma gondii infection. T. gondii infection induced activation of glycolysis, the Warburg effect, and disruption of the TCA cycle, with upregulation of genes such as Hif1a, Pkm, and Ldha. The Wnt/beta-catenin pathway, including genes like Ctnnb1 and Tnks, was also significantly elevated. XAV939 treatment mitigated these changes, suppressing glycolytic and Wnt/beta-catenin signaling pathways. KEGG enrichment highlighted infection-induced metabolic reprogramming and oxidative stress. XAV939 treatment mitigated these alterations, downregulating key glycolytic and Wnt/beta-catenin pathway genes. These findings underscore the parasite's impact on host immune and metabolic landscapes and highlight XAV939's therapeutic potential in modulating infection-induced dysregulation. This study provides critical insights into the molecular mechanisms underlying host-pathogen interactions and identifies potential targets for therapeutic intervention.