Calibration fluorescent bead dataset for imaging flow cytometry with bespoke parameters

Bead data were acquired using a BD FACSDiscover™ S8 Cell Sorter (BD Biosciences) operated with BD CellView™ software v6.2, a 100 μm nozzle, 20 psi sheath pressure and flow rate 1. BD FACS™ Accudrop Beads (BD Biosciences), comprising a uniform population of approximately 6μm diameter fluorescent particles, were used. The beads contain a fluorophore with excitation at 670 nm and emission at 750 nm.The folder contains:334 Image files for each event (.TIFF) with six distinct channels:1: Axial light loss2: Forward scatter3: Side scatter4-6: Three Fluorescence imaging channels which are band pass filtered (534/46nm, 600/60nm, 788/255nm)334 paired bespoke parameters derived from images:- Area of channel 2 (pixels in the event mask)- Spatial correlation between channels 2 and 6 (pixel-wise Pearson correlation)- Segmentation of channel 2 mask (number of unique connected components in the mask)