Limosilactobacillus reuteri B1-10 alleviates obesity in rats fed a high-fat diet by modulating the gut microbiota, metabolites, and inhibiting the JAK-STAT signalling pathway

In the present study, diet-induced specific pathogen-free obese rats were treated with different doses of breast milk-derived Limosilactobacillus reuteri B1-10. Treatment with L. reuteri B1-10 significantly reduced body weight and serum levels of triglycerides, total cholesterol, lipopolysaccharide, interleukin (IL)-10, and IL-1β. It also remodelled the gut microbiota composition. Additionally, L. reuteri B1-10 ameliorated the production of short-chain fatty acids and lipid and lipid-like molecules, mitigating associated disruptions in energy metabolism, protein synthesis, and amino acid pathways. Furthermore, L. reuteri B1-10 inhibited the JAK-STAT signaling pathway linked to inflammation.

本研究以饮食诱导的无特定病原体（specific pathogen-free，SPF）肥胖大鼠为实验对象，给予不同剂量的母乳源罗伊氏乳杆菌（Limosilactobacillus reuteri）B1-10进行干预。结果显示，罗伊氏乳杆菌B1-10可显著降低大鼠体重及血清甘油三酯、总胆固醇、脂多糖、白细胞介素（interleukin，IL）-10与IL-1β水平，同时重塑肠道菌群组成。此外，该菌株可改善短链脂肪酸及脂类、类脂分子的生成，缓解能量代谢、蛋白质合成与氨基酸通路相关的紊乱；进一步还可抑制与炎症相关的JAK-STAT信号通路。