snRNA-seq of the hippocampus of Mr1+/+ P301S mice and Mr1-/- P301S mice

We performed snRNA-seq using 10xgenomics platform to study the role of MAIT cells in regulating neuroinflammation in mice with neurodegenerative tau pathology. MAIT cells are a type of innate-like T cells that are present in the meninges of mice carrying neurodgenerative human tau mutant transgene. This is the first study to examine single nucleus transcriptome profiles of human tau mice lacking MAIT cells. Nuclei was extracted from 4 Mr1-/- P301S mice that lack MAIT cells and 4 control Mr1+/+ P301S mice. Single-nucleus RNA-seq was performed using 10xgenomics GEM 3' gene expression kit. Libraries were sequenced using a Novaseq.