A 24-Color Flow Cytometry Panel for Antigen-Specific T Cell Immunoprofiling and Functional Assessment of Human PBMCs in Vaccinated Individuals

The dataset contains original FCS files and related matrix files for the OMIP titled “A 24-Color Flow Cytometry Panel for Antigen-Specific T Cell Immunoprofiling and Functional Assessment of Human PBMCs in Vaccinated Individuals.” It includes FCS files from vaccinated recipient PBMCs used to generate Figure 1 in the manuscript. These files include unstimulated samples and samples stimulated with PMA/ionomycin, HIV-1 Env peptide pools, or HIV-1 Gag peptide pools. The dataset also includes single-stain PBMC compensation control FCS files used to establish the compensation matrix for the final 24-color panel. For the panel design and optimization analyses, the dataset includes FCS files used for stain index calculation, antibody titration, alternative fluorochrome-conjugate evaluation, candidate-marker assessment, and spillover-spreading matrix generation. It also includes the FCS files used for compensation-quality assessment and manual compensation evaluation, together with the final compensation matrix.