Gene expression changes in primary mouse fibroblasts in the absence of ASCIZ

In order to identify potential transcriptional targets of ASCIZ, we performed a microarray analysis of three wildltype and ASCIZ(ATMIN/ZNF822)-deficient littermate single-passage MEF cultures. Only 22 and 25 genes were found to be down- or upregulated in the KO, respectively, by >1.5-fold (p<0.05). The most severely downregulated gene was Dynll1, which was confirmed by Norhtern and Western blot in mulitple embryonic tissues. Primary MEFs were isolated from embryonic day 12.5 embryos (3 wildtype, 3 knockouts), and passaged once before isolation of total RNA.