Transcriptome profiling elucidates the blood-enriching effects and immune-regulation mechanism of steam-processed Polygonatum sibiricum polysaccharide in blood deficiency syndrome mice

Polygonatum sibiricum Red. has been used as a medicinal herb and nutritional food in traditional Chinese medicine for a long time. This study aimed to investigate the chemical properties, blood-enriching effects and mechanism of polysaccharide from the steam-processed Polygonatum sibiricum (SPS), which is a common form of Polygonatum sibiricum in clinical applications. RNA-seq analysis showed that 647 differentially expressed genes (DEGs) were obtained after SPSP treatment. GO and KEGG analysis revealed that SPSP-regulated DEGs were mainly involved in hematopoiesis, immune regulation signaling pathways. The reliability of transcriptome profiling was validated by quantitative real-time PCR.