The effect of hsa-miR-200c-3p/ZEB1 loop in the epithelial-mesenchymal transition of ovarian cancer

Ovarian cancer (OC) is the diagnosis of disease at advanced stages with distant metastasis described as the transition of cells from epithelial to mesenchymal phenotype (EMT).The expression of hsa-miR-200c-3p was analyzed on the ovarian tissues obtained from healthy individuals (n=15), and advanced epithelial OC (EOC) patients (n=15). ZEB1 is one of the target genes of hsa-miR-200c-3p and was determined by in-silico methods. The expression of ZEB1 was analyzed in both groups.The expression of hsa-miR-200c-3p was significantly increased in OC patients (p <0.05). ZEB1 expression level decreased significantly (p <0.05) in EOC group. The correlation analysis indicated a strong negative correlation between hsa-miR-200c-3p and the ZEB1 gene (r=-0,75; p<0,00).

卵巢癌（Ovarian Cancer, OC）是一类于晚期阶段确诊且伴远处转移的疾病，其肿瘤进展被定义为细胞从上皮表型向间质表型的转化过程，即上皮间质转化（Epithelial-Mesenchymal Transition, EMT）。本研究采集健康个体（n=15）与晚期上皮性卵巢癌（Epithelial Ovarian Cancer, EOC）患者（n=15）的卵巢组织，对其中人源微小RNA-200c-3p（hsa-miR-200c-3p）的表达水平进行了检测分析。锌指E盒结合同源盒1（ZEB1）是hsa-miR-200c-3p的靶基因之一，其靶向关联通过生物信息学（in-silico）方法预测得到。研究同时对两组样本中ZEB1的表达水平进行了检测。结果显示，OC患者体内hsa-miR-200c-3p的表达水平显著升高（p<0.05）；EOC组患者的ZEB1表达水平则显著降低（p<0.05）。相关性分析表明，hsa-miR-200c-3p与ZEB1基因的表达呈强负相关（r=-0.75；p<0.00）。