Differential gene expression between feminized C. elegans male and female germlines

With the aim to determine the extent of germline feminization in feminized male worms, we performed differential gene expression profiling of isolated gonads and whole worms between fog-1(q253) XO males and XX females. Overall design: mRNA-seq data sets were generated from fog-1(q253) XO males and XX females grown at the restrictive temperature. Both gonadal and whole worm YA samples were obtained.