Primers and probes for the 5′UTR and NS5B regions.
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*Reference sequence (GenBank, AF009606). M: A or C; Y: C or T; W: A or T; R: A or G. 5′UTR (untranslated region) specific primers: HCV1F (forward), HCVRX (reverse). NS5B (nonstructural 5B region) specific primers: NSNOF (forward), NSNOR (reverse). Modification: Forward primer had 5′ biotin added for streptavidin-R-phycoerythrin (SA-PE) ligation. Probes had 5′ amino-12C (carbon linker) added for ligation to the microspheres.
创建时间：
2010-09-20
